Near-infrared spectroscopy for monitoring... - ERP Spain.com - España y América Latina

Full Title: Near-infrared spectroscopy for monitoring Maillard reaction and critical process parameters of a C. tetani bioprocess Purpose: The goal of the study is to determine if near-infrared spectroscopy (NIR) is a feasible analytical method to monitor in-line process parameters for C. tetani bioprocess monitoring and Maillard reaction. Methods: Off-line NIR measurements were acquired using the Matrix-F and Excalibur XP25 probe or the Multi-Purpose Analyzer (MPA) Fourier Transform-NIR (FT-NIR) spectrometer. All in-line measurements were taken using the Matrix-F spectrometer and Excalibur XP25 probe. The C. tetani fermentation samples used to build the calibration models were from various 20L fermenters, and multivariate models were used to evaluate critical process parameters to determine feasibility of measurement using reference standards. Results: Cross-leave-one out validation models were able to predict critical process parameters including pH, optical density (OD), total protein concentration and glucose off-line during fermentation, as determined by the goodness of fit (R2) and Root Mean Square Error of Cross Validation (RMSECV) values to the standard reference methods. Off-line NIR calibration models for the process parameters were unable to predict in-line C. tetani fermentation spectra. PCA cluster analysis showed distinct clusters between in-line NIR spectra for various temperatures treatments to monitor Maillard reaction in media samples prior to bacterial inoculation. Conclusion: Off-line calibration models were able to monitor process parameters involved in Tetanus upstream fermentation including pH, OD, total protein concentration and glucose. Furthermore, in-line PCA analysis demonstrated feasible monitoring of heat-treated media and distinct clusters involved in Maillard browning.