In Depth Characterization with Mass Spectrometry and Orthogonal Methods of... - ERP Spain.com - España y América Latina

Full Title: In Depth Characterization with Mass Spectrometry and Orthogonal Methods of Conformers in a Trispecific HIV Antibody Presented by Bruno Genet, Lab head Structural Analysis MS at Sanofi The initial characterization of a trispecific anti-HIV antibody lead to an aberrant two-peaks size exclusion chromatography profile. In such case, a column interaction is suspected, but a possibility of actually having conformers was taken into consideration. We have used both analytical characterization, structural analysis and molecular dynamics to cross all the informations. The mass spectrometry toolbox has been largely used with native and denaturing MS, ion mobility and direct infusion to carefully study both the antibody and the antigens interactions.